Description
General Description
NPC-PEG-NPC is a homobifunctional polyethylene glycol derivative containing p-nitrophenyl carbonate (NPC) groups at both PEG termini.
The terminal NPC groups are amine-reactive activated carbonates that can react with primary amines (NH₂), including amine-containing proteins, peptides, and other biomolecules, to form stable carbamate linkages.
Because NPC groups are present at both ends, NPC-PEG-NPC can serve as a bifunctional PEG spacer or crosslinker for connecting two amine-containing molecules while introducing a hydrophilic and flexible PEG segment.
NSP offers NPC-PEG-NPC with different PEG molecular weights for bioconjugation, PEGylation, crosslinking, biomolecule modification, and surface functionalization.
Applications
- Protein PEGylation
- Peptide PEGylation
- Bioconjugation
- Molecular crosslinking
- Protein and peptide modification
- Biomolecule immobilization
- Antibody modification
- Nanoparticle functionalization
- Surface modification
- Hydrogel and biomaterial crosslinking
- Synthesis of PEG conjugates
Features and Benefits
- NPC groups at both PEG termini
- Homobifunctional PEG linker
- Two amine-reactive activated carbonate groups
- Reacts with primary amines
- Forms stable carbamate linkages
- Hydrophilic and flexible PEG spacer
- Suitable for protein and peptide PEGylation
- Suitable for crosslinking applications
- Multiple PEG molecular weights available
- Custom molecular weights available
Conjugation Chemistry
The terminal p-nitrophenyl carbonate (NPC) groups react with primary amines to form stable carbamate linkages with release of p-nitrophenol as the leaving group. Primary amines on proteins, peptides, antibodies, amine-functionalized polymers, nanoparticles, and surfaces can be coupled with NPC-PEG-NPC. Because activated carbonate groups are present at both PEG termini, NPC-PEG-NPC can function as a homobifunctional crosslinker, connecting two amine-containing molecules through a PEG spacer.
