Description
General Description
Biotin-PEG4-Azide (Biotin-PEG4-Nā) is a biotin-containing click chemistry linker consisting of a biotin moiety, a defined PEG4 spacer, and a terminal azide (Nā) functional group.
The terminal azide reacts with terminal alkynes through copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or with strained cyclooctynes such as DBCO and BCN through strain-promoted azide-alkyne cycloaddition (SPAAC).
The biotin moiety provides strong noncovalent binding to avidin, streptavidin, and neutravidin. This enables click-labeled biomolecules to be detected, captured, purified, or immobilized using biotin-binding proteins.
The defined PEG4 spacer provides hydrophilic separation between biotin and the azide functionality, improving accessibility while maintaining a precisely defined linker structure.
Applications
- Biotinylation
- CuAAC click chemistry
- Copper-free SPAAC click chemistry
- Affinity labeling
- Protein and peptide modification
- Biomolecule immobilization
- Streptavidin-based detection
- Affinity capture and purification
- Nanoparticle functionalization
- Surface modification
- Molecular probe development
Features and Benefits
- Biotin functionality
- Terminal azide (Nā)
- Defined PEG4 spacer
- Compatible with CuAAC
- Compatible with copper-free SPAAC
- Reactive with terminal alkynes, DBCO, and BCN
- Strong biotin-streptavidin interaction
- Hydrophilic PEG spacer
- Defined molecular structure
- Suitable for affinity capture and detection
- Useful for bioorthogonal bioconjugation
