Description
General Description
Azide-dPEG23-Amine (N₃-dPEG23-NH₂) is a heterobifunctional discrete polyethylene glycol linker containing an azide (N₃) at one end and a primary amine (NH₂) at the other, separated by a defined PEG23 spacer.
Unlike conventional polydisperse PEG polymers, dPEG linkers have a defined chain length and molecular structure. The relatively long PEG23 spacer provides substantial hydrophilic and flexible separation between the two reactive termini, which can be advantageous when conjugating large biomolecules, nanoparticles, surfaces, or sterically hindered functional groups.
The terminal azide participates in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal alkynes and strain-promoted azide-alkyne cycloaddition (SPAAC) with strained cyclooctynes such as DBCO and BCN.
The primary amine provides an independent reactive site for NHS esters, activated carboxylic acids, aldehydes, and other amine-reactive compounds. The combination of azide and amine functionalities enables orthogonal and sequential conjugation strategies.
Applications
- CuAAC click chemistry
- Copper-free SPAAC click chemistry
- Bioconjugation
- Protein and peptide modification
- Antibody conjugation
- Drug conjugation
- Long-spacer linker synthesis
- Fluorescent probe conjugation
- Nanoparticle functionalization
- Surface modification
- Biomolecule immobilization
- PROTAC and molecular linker research
Features and Benefits
- Defined dPEG23 spacer
- Heterobifunctional azide and amine termini
- Long, hydrophilic PEG spacer
- Increased spatial separation between reactive groups
- Compatible with CuAAC
- Compatible with copper-free SPAAC
- Primary amine for NHS ester coupling
- Suitable for orthogonal conjugation
- Defined molecular structure
- Useful for biomolecule and nanoparticle conjugation
