Description
General Description
Azide-dPEG35-Amine (N₃-dPEG35-NH₂) is a heterobifunctional discrete polyethylene glycol linker containing an azide (N₃) at one end and a primary amine (NH₂) at the other, separated by a defined PEG35 spacer.
Unlike conventional polydisperse PEG polymers, dPEG linkers have a defined chain length and molecular structure. The long PEG35 spacer provides substantial hydrophilic and flexible separation between the two reactive termini. This extended spacer is particularly useful when additional distance is desired between conjugated biomolecules, nanoparticles, surfaces, or sterically hindered functional groups.
The terminal azide participates in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal alkynes and strain-promoted azide-alkyne cycloaddition (SPAAC) with strained cyclooctynes such as DBCO and BCN.
The primary amine provides an independent reactive site for NHS esters, activated carboxylic acids, aldehydes, and other amine-reactive compounds. The two different terminal functionalities enable orthogonal and sequential conjugation strategies.
Applications
- CuAAC click chemistry
- Copper-free SPAAC click chemistry
- Bioconjugation
- Protein and peptide modification
- Antibody conjugation
- Drug conjugation
- Long-spacer linker synthesis
- Biomolecule immobilization
- Nanoparticle functionalization
- Surface modification
- Molecular probe development
- PROTAC and molecular linker research
Features and Benefits
- Defined dPEG35 spacer
- Heterobifunctional azide and amine termini
- Long, hydrophilic PEG spacer
- Increased spatial separation between reactive groups
- Compatible with CuAAC
- Compatible with copper-free SPAAC
- Primary amine for NHS ester coupling
- Suitable for orthogonal conjugation
- Defined molecular structure
- Useful for biomolecule, nanoparticle, and surface conjugation
