Description
General Description
Biotin-PEG2-Maleimide is a heterobifunctional biotinylation reagent consisting of a biotin moiety, a defined PEG2 spacer, and a terminal maleimide functional group.
The maleimide group reacts efficiently with thiol (SH) groups, particularly cysteine residues in peptides and proteins, through Michael addition to form a covalent thioether linkage.
The biotin moiety provides strong noncovalent binding to avidin, streptavidin, and neutravidin, allowing the conjugated biomolecule to be detected, captured, purified, or immobilized using biotin-binding proteins.
The defined PEG2 spacer provides hydrophilic separation between the biotin group and the conjugated molecule while maintaining a compact linker structure.
Applications
- Protein biotinylation
- Peptide biotinylation
- Cysteine-selective labeling
- Thiol-maleimide conjugation
- Antibody modification
- Affinity labeling
- Bioconjugation
- Streptavidin-based detection
- Affinity capture and purification
- Biomolecule immobilization
- Surface functionalization
- Molecular probe development
Features and Benefits
- Biotin functionality
- Thiol-reactive maleimide group
- Defined PEG2 spacer
- Suitable for cysteine-containing proteins and peptides
- Strong biotin–streptavidin interaction
- Hydrophilic PEG spacer
- Defined molecular structure
- Suitable for affinity capture and detection
- Useful for protein and peptide biotinylation
- Research-grade bioconjugation reagent
