Description
General Description
DSPE-PEG-Tetrazine (DSPE-PEG-Tz) is a heterobifunctional lipid-PEG derivative composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), a polyethylene glycol (PEG) spacer, and a terminal tetrazine (Tz) functionality.
The hydrophobic DSPE moiety provides a lipid anchor that enables incorporation into liposomes, lipid nanoparticles (LNPs), micelles, and other lipid-based nanostructures. The hydrophilic PEG spacer extends the reactive tetrazine group away from the lipid surface while providing steric stabilization and improved aqueous dispersibility.
Tetrazine undergoes an exceptionally rapid and selective inverse electron-demand Diels–Alder (IEDDA) reaction with strained dienophiles, particularly trans-cyclooctene (TCO). This bioorthogonal click reaction proceeds under mild conditions without copper or other catalysts, making DSPE-PEG-Tetrazine particularly useful for biological conjugation and nanomedicine applications.
DSPE-PEG-Tetrazine can be used to functionalize lipid-based nanoparticles with TCO-modified peptides, proteins, antibodies, targeting ligands, fluorophores, and other biomolecules. It is also useful for pretargeting strategies, molecular imaging, diagnostic systems, and advanced drug delivery platforms.
Applications
- Tetrazine-TCO click chemistry
- IEDDA bioorthogonal conjugation
- Liposome functionalization
- Lipid nanoparticle (LNP) modification
- Antibody conjugation
- Peptide conjugation
- Targeting ligand attachment
- Targeted drug delivery
- Molecular imaging
- Nanomedicine and biomaterials research
Features and Benefits
- DSPE lipid anchor
- Hydrophilic PEG spacer
- Terminal tetrazine functionality
- Highly reactive toward TCO
- Fast IEDDA click chemistry
- Copper-free and catalyst-free conjugation
- Suitable for bioorthogonal labeling
- Compatible with lipid nanoparticles and liposomes
- Excellent aqueous dispersibility
- Research-grade quality
