Description
General Description
Tetrazine-Ph-NHS Ester is a bifunctional bioconjugation reagent designed for introducing tetrazine functionality onto amine-containing biomolecules and materials.
The NHS ester reacts efficiently with primary amines, including lysine side chains and N-terminal amino groups of proteins and peptides, forming stable amide bonds. This provides a convenient approach for preparing tetrazine-functionalized proteins, peptides, antibodies, polymers, nanoparticles, and surfaces.
The tetrazine group undergoes a rapid and highly selective inverse electron-demand Diels–Alder (IEDDA) reaction with strained dienophiles, particularly trans-cyclooctene (TCO). The reaction proceeds without copper or other catalysts and is widely used for bioorthogonal labeling and conjugation.
Tetrazine-Ph-NHS Ester is particularly useful for protein labeling, antibody modification, nanoparticle functionalization, molecular imaging, targeted drug delivery, diagnostics, and biomaterials research.
Applications
- Protein tetrazine labeling
- Peptide modification
- Antibody conjugation
- Amine-selective functionalization
- Tetrazine-TCO click chemistry
- IEDDA bioorthogonal conjugation
- Nanoparticle functionalization
- Molecular imaging
- Targeted drug delivery
- Biomaterials research
Features and Benefits
- Amine-reactive NHS ester
- Phenyl-tetrazine functionality
- Rapid reaction with TCO
- Highly selective IEDDA chemistry
- Copper-free click chemistry
- Catalyst-free bioorthogonal conjugation
- Suitable for proteins, peptides, and antibodies
- Convenient introduction of tetrazine groups
