Description
General Description
Cy3-NHS Ester is a widely used amine-reactive fluorescent dye for covalent labeling of biomolecules. The Cyanine 3 fluorophore provides bright orange-red fluorescence with excitation and emission maxima of approximately 550 nm and 570 nm, respectively. Its high quantum yield, excellent brightness, and good photostability make it a preferred fluorophore for biological imaging and analytical assays.
The activated NHS ester reacts rapidly with primary amines under mild aqueous conditions (typically pH 7.2–8.8), forming stable amide bonds with lysine residues on proteins, peptides, antibodies, enzymes, amino-modified oligonucleotides, polymers, nanoparticles, and other biomolecules.
Cy3-NHS Ester is extensively employed in fluorescence microscopy, immunofluorescence, flow cytometry, FRET studies, biosensor development, nanoparticle labeling, molecular diagnostics, and drug delivery research.
Applications
- Protein labeling
- Antibody labeling
- Peptide labeling
- Oligonucleotide labeling
- Polymer labeling
- Nanoparticle labeling
- Fluorescence microscopy
- Flow cytometry
- FRET assays
- Molecular diagnostics
Features and Benefits
- Bright orange-red fluorescence
- High quantum yield
- Excellent photostability
- Reactive NHS ester
- Rapid amine-selective conjugation
- Stable amide bond formation
- Suitable for multicolor imaging
- Research-grade quality
