Description
General Description
Cy7-NHS Ester is a premium amine-reactive fluorescent dye designed for covalent labeling of biomolecules with a bright near-infrared fluorophore. The Cyanine 7 (Cy7) dye exhibits excitation and emission maxima of approximately 750 nm and 775 nm, respectively, providing excellent tissue penetration, minimal biological autofluorescence, and a high signal-to-background ratio for biological imaging applications.
The activated NHS ester reacts rapidly with primary amines under mild aqueous conditions (typically pH 7.2–8.8), forming stable amide bonds with lysine residues on proteins, peptides, antibodies, enzymes, amino-modified oligonucleotides, polymers, nanoparticles, and other amine-containing biomolecules.
Because of its long-wavelength fluorescence, Cy7-NHS Ester is particularly well suited for in vivo optical imaging, fluorescence-guided surgery, biodistribution studies, targeted drug delivery, nanoparticle tracking, multiplex fluorescence assays, and molecular diagnostics.
Applications
- Protein labeling
- Antibody labeling
- Peptide labeling
- Oligonucleotide labeling
- Polymer labeling
- Nanoparticle labeling
- Near-infrared fluorescence imaging
- In vivo imaging
- Flow cytometry
- Molecular diagnostics
Features and Benefits
- Bright near-infrared fluorescence
- Excellent tissue penetration
- Low biological autofluorescence
- High signal-to-background ratio
- Reactive NHS ester
- Rapid amine-selective conjugation
- Stable amide bond formation
- Research-grade quality
