Description
General Description
Azide-dPEG5-Amine (N₃-dPEG5-NH₂) is a heterobifunctional discrete polyethylene glycol linker containing an azide (N₃) at one end and a primary amine (NH₂) at the other, separated by a defined PEG5 spacer.
Unlike conventional polydisperse PEG polymers, dPEG linkers have a defined molecular structure and chain length. The PEG5 spacer provides hydrophilicity and spatial separation between the two reactive termini.
The azide functionality participates in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal alkynes and strain-promoted azide-alkyne cycloaddition (SPAAC) with strained cyclooctynes such as DBCO and BCN.
The primary amine can react with NHS esters, activated carboxylic acids, aldehydes, and other amine-reactive compounds. The two different terminal functionalities enable orthogonal and sequential conjugation strategies.
Applications
- CuAAC click chemistry
- Copper-free SPAAC click chemistry
- Bioconjugation
- Protein and peptide modification
- Antibody conjugation
- Drug conjugation
- Linker synthesis
- Fluorescent probe conjugation
- Nanoparticle functionalization
- Surface modification
- PROTAC and molecular linker research
Features and Benefits
- Defined dPEG5 spacer
- Heterobifunctional azide and amine termini
- Compatible with CuAAC
- Compatible with copper-free SPAAC
- Primary amine for NHS ester coupling
- Hydrophilic PEG spacer
- Suitable for orthogonal conjugation
- Defined molecular structure
- Useful for multistep bioconjugation
