Description
General Description
DBCO-PEG-COOH is a versatile heterobifunctional PEG linker designed for bioorthogonal conjugation and polymer functionalization. The terminal DBCO group readily reacts with azides through strain-promoted azide-alkyne cycloaddition (SPAAC), a copper-free click chemistry reaction that proceeds rapidly under physiological conditions without the need for a copper catalyst. This makes DBCO-PEG-COOH particularly suitable for live-cell labeling, in vivo imaging, and other applications where copper catalysts are undesirable.
The terminal carboxylic acid group can be activated using carbodiimide chemistry (EDC/NHS) or other coupling reagents to react with primary amines on proteins, peptides, antibodies, polymers, nanoparticles, and biomaterial surfaces, forming stable amide bonds.
The hydrophilic PEG spacer improves water solubility, molecular flexibility, and biocompatibility while reducing steric hindrance and minimizing nonspecific protein adsorption. DBCO-PEG-COOH is extensively employed in click chemistry, antibody conjugation, nanoparticle engineering, hydrogel synthesis, targeted drug delivery, and advanced biomaterials development.
Applications
- Copper-free click chemistry (SPAAC)
- Protein conjugation
- Antibody conjugation
- Peptide conjugation
- Surface modification
- Nanoparticle functionalization
- Hydrogel synthesis
- Drug delivery systems
- Molecular imaging
- Biomaterials research
Features and Benefits
- Reactive DBCO functionality
- Terminal carboxylic acid group
- Copper-free click chemistry
- Compatible with EDC/NHS coupling
- Rapid azide conjugation
- Excellent water solubility
- Excellent biocompatibility
- Low nonspecific protein adsorption
- Available in multiple PEG molecular weights
