Description
General Description
Azide-dPEG2-Amine (N₃-dPEG2-NH₂) is a heterobifunctional discrete polyethylene glycol linker containing an azide (N₃) at one end and a primary amine (NH₂) at the other, separated by a defined PEG2 spacer.
Unlike conventional polydisperse PEG polymers, dPEG linkers have a defined chain length and molecular structure. The PEG2 spacer provides a short hydrophilic linker with controlled spacing between the two reactive termini.
The terminal azide participates in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal alkynes and strain-promoted azide-alkyne cycloaddition (SPAAC) with strained cyclooctynes such as DBCO and BCN.
The primary amine provides an independent reactive site for NHS esters, activated carboxylic acids, aldehydes, and other amine-reactive compounds. The combination of azide and amine functionalities enables orthogonal and sequential conjugation strategies.
Applications
- CuAAC click chemistry
- Copper-free SPAAC click chemistry
- Bioconjugation
- Protein and peptide modification
- Antibody conjugation
- Drug conjugation
- Linker synthesis
- Fluorescent probe conjugation
- Nanoparticle functionalization
- Surface modification
- PROTAC and molecular linker research
Features and Benefits
- Defined dPEG2 spacer
- Heterobifunctional azide and amine termini
- Compatible with CuAAC
- Compatible with copper-free SPAAC
- Primary amine for NHS ester coupling
- Short hydrophilic PEG spacer
- Suitable for orthogonal conjugation
- Defined molecular structure
- Useful for multistep bioconjugation
