Description
General Description
OPSS-Poly(L-Lysine) is a functional cationic polypeptide designed for reversible thiol conjugation and advanced drug delivery applications. The terminal OPSS (ortho-pyridyl disulfide) group reacts rapidly and selectively with free sulfhydryl (thiol) groups on proteins, peptides, antibodies, and other biomolecules through thiol-disulfide exchange, forming cleavable disulfide linkages while releasing pyridine-2-thione.
The poly(L-lysine) backbone contains numerous primary amine groups that readily complex with negatively charged nucleic acids such as DNA, RNA, siRNA, and mRNA. These amine groups also react with NHS esters, activated carboxylic acids, aldehydes, epoxides, and other amine-reactive molecules, providing versatile opportunities for additional functionalization.
The combination of a cationic biodegradable polypeptide and a terminal OPSS group makes OPSS-PLL an excellent material for redox-responsive gene delivery systems, polymer-drug conjugates, reversible protein conjugation, nanoparticle functionalization, hydrogel preparation, and advanced biomaterials.
Applications
- Reversible thiol conjugation
- Gene delivery
- DNA and RNA complexation
- Protein conjugation
- Antibody conjugation
- Nanoparticle functionalization
- Surface modification
- Hydrogel synthesis
- Redox-responsive drug delivery
- Biomaterials research
Features and Benefits
- Terminal OPSS functionality
- Selective thiol-disulfide exchange
- Forms cleavable disulfide linkages
- Multiple primary amine groups
- Excellent nucleic acid binding
- Excellent aqueous solubility
- Biodegradable polypeptide
- Available in multiple molecular weights
