Description
General Description
HO-PEG-NHS is a versatile heterobifunctional PEG linker designed for selective amine conjugation and polymer functionalization. The terminal NHS ester readily reacts with primary amines on proteins, peptides, antibodies, enzymes, nanoparticles, and other biomolecules under mild aqueous conditions, forming stable amide linkages with high efficiency.
The terminal hydroxyl group serves as a convenient synthetic handle and can be converted into a variety of functional groups, including carboxyl, amine, aldehyde, azide, alkyne, maleimide, acrylate, methacrylate, epoxide, and thiol. This makes HO-PEG-NHS an important intermediate for the synthesis of customized PEG derivatives and multifunctional biomaterials.
The hydrophilic PEG spacer enhances water solubility, molecular flexibility, and biocompatibility while minimizing steric hindrance and reducing nonspecific protein adsorption. HO-PEG-NHS is widely employed in PEGylation, protein labeling, hydrogel synthesis, nanoparticle engineering, surface modification, and targeted drug delivery.
Applications
- Protein conjugation
- Antibody conjugation
- Peptide conjugation
- PEGylation
- Polymer synthesis
- Surface modification
- Nanoparticle functionalization
- Hydrogel synthesis
- Drug delivery systems
- Biomaterials research
Features and Benefits
- Heterobifunctional PEG linker
- Reactive NHS ester
- Terminal hydroxyl group
- Efficient amine-specific conjugation
- Forms stable amide bonds
- Excellent water solubility
- Excellent biocompatibility
- Versatile synthetic intermediate
- Available in multiple PEG molecular weights
