Description
General Description
mPEG-Maleimide is a highly reactive monofunctional PEG reagent designed for site-selective thiol conjugation and PEGylation applications. The terminal methoxy group is chemically inert, preventing polymer crosslinking, while the terminal maleimide group selectively reacts with free sulfhydryl groups on cysteine-containing proteins, peptides, antibodies, enzymes, and other thiolated biomolecules.
The maleimide-thiol reaction proceeds rapidly under mild aqueous conditions (typically pH 6.5–7.5), forming stable thioether bonds with high efficiency and selectivity. This chemistry is widely employed for site-specific PEGylation, antibody-drug conjugates (ADCs), peptide conjugation, nanoparticle functionalization, and targeted drug delivery.
The PEG spacer enhances aqueous solubility, improves biocompatibility, reduces nonspecific protein adsorption, and prolongs circulation time in vivo. mPEG-MAL is one of the most widely used functional PEG reagents in pharmaceutical, biotechnology, and nanomedicine applications.
Applications
- Thiol-selective PEGylation
- Protein conjugation
- Peptide conjugation
- Antibody modification
- Enzyme modification
- Nanoparticle functionalization
- Surface modification
- Drug delivery systems
- Antibody-drug conjugates (ADCs)
- Biomaterials research
Features and Benefits
- Monofunctional PEG reagent
- Reactive maleimide functionality
- Rapid thiol-selective conjugation
- Stable thioether bond formation
- Excellent water solubility
- Excellent biocompatibility
- Reduces nonspecific protein adsorption
- Available in multiple PEG molecular weights
