Description
General Description
mPEG-Thiol (mPEG-SH) is a highly versatile monofunctional PEG reagent designed for selective thiol conjugation and PEGylation applications. The terminal methoxy group is chemically inert, preventing polymer crosslinking, while the terminal thiol group provides a highly reactive sulfhydryl functionality for a broad range of bioconjugation reactions.
The thiol group reacts rapidly with maleimides to form stable thioether bonds and also participates in thiol-disulfide exchange, thiol-ene click reactions, Michael addition, and conjugation with haloacetyl-functionalized molecules. Additionally, the thiol group exhibits strong affinity toward gold surfaces, enabling the preparation of self-assembled monolayers and functionalized gold nanoparticles.
The PEG spacer enhances aqueous solubility, improves biocompatibility, reduces nonspecific protein adsorption, and prolongs circulation time in vivo. mPEG-SH is widely used for protein PEGylation, nanoparticle engineering, hydrogel synthesis, biosensor fabrication, targeted drug delivery, and advanced biomedical research.
Applications
- Thiol-selective conjugation
- Protein PEGylation
- Peptide conjugation
- Nanoparticle functionalization
- Gold nanoparticle modification
- Surface modification
- Hydrogel synthesis
- Thiol-ene click chemistry
- Drug delivery systems
- Biomaterials research
Features and Benefits
- Linear monofunctional PEG reagent
- Terminal thiol functionality
- Compatible with maleimide conjugation
- Compatible with thiol-disulfide exchange
- Compatible with thiol-ene chemistry
- Gold surface immobilization
- Excellent water solubility
- Available in multiple PEG molecular weights
