Description
General Description
mPEG-Iodoacetyl is a highly reactive monofunctional PEG reagent designed for selective thiol conjugation. The terminal methoxy group is chemically inert, preventing polymer crosslinking, while the terminal iodoacetyl group serves as an electrophilic handle that reacts readily with free sulfhydryl groups on cysteine-containing proteins, peptides, antibodies, enzymes, and other thiolated biomolecules.
The conjugation proceeds through nucleophilic substitution to form a stable thioether bond, providing an irreversible linkage with excellent stability under physiological conditions. Compared with maleimide chemistry, iodoacetyl reagents generally produce thioether bonds that are less susceptible to hydrolysis or exchange reactions.
The PEG spacer enhances aqueous solubility, improves biocompatibility, reduces nonspecific protein adsorption, and prolongs circulation time in vivo. mPEG-Iodoacetyl is widely used in therapeutic protein PEGylation, antibody modification, nanoparticle surface engineering, biosensor fabrication, and advanced biomedical research.
Applications
- Thiol-selective PEGylation
- Protein conjugation
- Peptide conjugation
- Antibody modification
- Enzyme modification
- Nanoparticle functionalization
- Surface modification
- Biosensor fabrication
- Drug delivery systems
- Biomaterials research
Features and Benefits
- Monofunctional PEG reagent
- Reactive iodoacetyl functionality
- Selective thiol conjugation
- Stable thioether bond formation
- Excellent water solubility
- Excellent biocompatibility
- Reduces nonspecific protein adsorption
- Available in multiple PEG molecular weights
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