Description
General Description
NH2-PEG-COOH (Amine-PEG-Carboxyl) is a versatile heterobifunctional PEG linker designed for orthogonal bioconjugation and polymer functionalization. The terminal primary amine reacts efficiently with NHS esters, activated carboxylic acids, aldehydes, isothiocyanates, epoxides, and other electrophilic functional groups, allowing selective conjugation to proteins, peptides, polymers, nanoparticles, and biomaterial surfaces.
The terminal carboxylic acid group can be activated using carbodiimide chemistry (EDC/NHS) or peptide coupling reagents such as HATU or HBTU for reaction with primary amines, providing an additional orthogonal conjugation site. The presence of both amino and carboxyl functionalities makes NH2-PEG-COOH an excellent intermediate for the synthesis of multifunctional PEG derivatives.
The hydrophilic PEG spacer enhances water solubility, molecular flexibility, and biocompatibility while minimizing steric hindrance and reducing nonspecific protein adsorption. NH2-PEG-COOH is extensively used in protein conjugation, peptide synthesis, hydrogel preparation, nanoparticle engineering, targeted drug delivery, and advanced biomaterials development.
Applications
- Protein conjugation
- Peptide synthesis
- Polymer synthesis
- PEG linker synthesis
- Surface modification
- Nanoparticle functionalization
- Hydrogel synthesis
- Drug delivery systems
- Biomaterials engineering
- Biomedical research
Features and Benefits
- Terminal primary amine
- Terminal carboxylic acid group
- Compatible with EDC/NHS coupling
- Efficient amine conjugation
- Orthogonal functionalization
- Excellent water solubility
- Excellent biocompatibility
- Versatile synthetic intermediate
- Available in multiple PEG molecular weights
