Description
General Description
OPSS-PEG-NH2 is a versatile heterobifunctional PEG linker designed for orthogonal bioconjugation and redox-responsive biomaterials. The terminal OPSS (ortho-pyridyl disulfide) group reacts rapidly and selectively with free sulfhydryl (thiol) groups on cysteine-containing proteins, peptides, antibodies, enzymes, and other biomolecules through thiol-disulfide exchange, producing reversible disulfide bonds while releasing pyridine-2-thione.
The terminal primary amine reacts efficiently with activated carboxylic acids through EDC/NHS coupling, as well as NHS esters, aldehydes, epoxides, and isothiocyanates, enabling straightforward conjugation to proteins, peptides, polymers, nanoparticles, and biomaterial surfaces.
The hydrophilic PEG spacer improves water solubility, molecular flexibility, and biocompatibility while minimizing steric hindrance and reducing nonspecific protein adsorption. OPSS-PEG-NH2 is extensively used in protein conjugation, antibody modification, hydrogel synthesis, nanoparticle engineering, biosensor fabrication, targeted drug delivery, and stimuli-responsive biomaterials.
Applications
- Thiol-specific bioconjugation
- Reversible protein conjugation
- Antibody conjugation
- Peptide conjugation
- Nanoparticle functionalization
- Surface modification
- Hydrogel synthesis
- Drug delivery systems
- Redox-responsive biomaterials
- Biomaterials research
Features and Benefits
- Reactive OPSS (ortho-pyridyl disulfide) group
- Terminal primary amine
- Selective thiol-disulfide exchange
- Efficient amine conjugation
- Forms cleavable disulfide linkages
- Excellent water solubility
- Excellent biocompatibility
- Reduced nonspecific protein adsorption
- Available in multiple PEG molecular weights
