Description
General Description
OPSS-PEG-NHS is a versatile heterobifunctional PEG linker designed for orthogonal and reversible bioconjugation. The terminal NHS ester efficiently reacts with primary amines on proteins, peptides, antibodies, enzymes, nanoparticles, and other biomolecules under mild aqueous conditions, forming stable amide linkages.
The terminal OPSS (ortho-pyridyl disulfide) group reacts rapidly and selectively with free sulfhydryl (thiol) groups through thiol-disulfide exchange, producing reversible disulfide bonds while releasing pyridine-2-thione as the reaction by-product. The resulting disulfide linkage can be cleaved under reducing intracellular conditions, making OPSS-PEG-NHS particularly valuable for preparing redox-responsive drug delivery systems and cleavable bioconjugates.
The hydrophilic PEG spacer enhances water solubility, molecular flexibility, and biocompatibility while reducing steric hindrance and minimizing nonspecific protein adsorption. OPSS-PEG-NHS is widely employed in protein conjugation, antibody modification, hydrogel synthesis, nanoparticle functionalization, targeted drug delivery, biosensor fabrication, and advanced biomaterials development.
Applications
- Thiol-specific bioconjugation
- Reversible protein conjugation
- Antibody conjugation
- Peptide conjugation
- Drug delivery systems
- Nanoparticle functionalization
- Surface modification
- Hydrogel synthesis
- Redox-responsive biomaterials
- Biomaterials research
Features and Benefits
- Reactive OPSS (ortho-pyridyl disulfide) group
- Reactive NHS ester
- Selective thiol-disulfide exchange
- Efficient amine-specific conjugation
- Forms cleavable disulfide linkages
- Excellent water solubility
- Excellent biocompatibility
- Reduced nonspecific protein adsorption
- Available in multiple PEG molecular weights
