Description
General Description
RhB-PEG-NHSÂ (Rhodamine B-PEG-NHS) is a versatile fluorescent PEG linker designed for efficient amine-specific bioconjugation. The terminal NHS ester reacts readily with primary amines on proteins, peptides, antibodies, enzymes, nanoparticles, and other biomolecules under mild aqueous conditions, forming stable amide bonds with high labeling efficiency.
The Rhodamine B fluorophore emits intense red fluorescence with an excitation wavelength of approximately 540–560 nm and an emission wavelength of approximately 570–590 nm, making it ideal for fluorescence microscopy, confocal microscopy, flow cytometry, fluorescence spectroscopy, and multicolor imaging applications.
The hydrophilic PEG spacer improves water solubility, molecular flexibility, and biocompatibility while reducing steric hindrance and minimizing nonspecific protein adsorption. RhB-PEG-NHS is extensively employed in fluorescent protein labeling, antibody conjugation, nanoparticle functionalization, biosensor fabrication, molecular imaging, targeted drug delivery, and advanced biomaterials development.
Applications
- Protein labeling
- Antibody labeling
- Peptide conjugation
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Molecular imaging
- Nanoparticle functionalization
- Biosensor fabrication
- Biomaterials research
Features and Benefits
- Bright Rhodamine B fluorescence
- Reactive NHS ester
- Efficient amine-specific conjugation
- Forms stable amide bonds
- Excellent water solubility
- Excellent biocompatibility
- Reduced steric hindrance
- Low nonspecific protein adsorption
- Available in multiple PEG molecular weights
