Description
General Description
Maleimide-PEG-RhB is a multifunctional fluorescent PEG linker designed for thiol-specific bioconjugation and fluorescence imaging. The terminal maleimide group rapidly and selectively reacts with sulfhydryl (thiol) groups on cysteine-containing peptides, proteins, antibodies, enzymes, and other biomolecules under mild physiological conditions, forming stable thioether linkages.
The Rhodamine B fluorophore provides intense red fluorescence with an excitation wavelength of approximately 540–560 nm and an emission wavelength of approximately 570–590 nm, making it well suited for fluorescence microscopy, confocal microscopy, flow cytometry, fluorescence spectroscopy, and multicolor imaging applications.
The hydrophilic PEG spacer enhances water solubility, molecular flexibility, and biocompatibility while reducing steric hindrance and minimizing nonspecific protein adsorption. Maleimide-PEG-RhB is extensively used for fluorescent protein labeling, antibody labeling, nanoparticle functionalization, biosensor fabrication, molecular imaging, targeted drug delivery, and nanomedicine research.
Applications
- Thiol-specific protein labeling
- Antibody conjugation
- Peptide conjugation
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Molecular imaging
- Nanoparticle functionalization
- Biosensor fabrication
- Biomaterials research
Features and Benefits
- Reactive maleimide group
- Bright Rhodamine B fluorescence
- Highly selective thiol conjugation
- Forms stable thioether bonds
- Excellent water solubility
- Excellent biocompatibility
- Reduced steric hindrance
- Low nonspecific protein adsorption
- Available in multiple PEG molecular weights
