Description
General Description
mPEG-Vinyl Sulfone (mPEG-VS) is a highly reactive linear monofunctional PEG reagent designed for selective bioconjugation and PEGylation. The terminal methoxy group is chemically inert, preventing polymer crosslinking, while the vinyl sulfone group serves as a Michael acceptor that reacts efficiently with nucleophiles.
Under physiological conditions, the vinyl sulfone group reacts rapidly and selectively with thiol (sulfhydryl) groups on cysteine-containing proteins, peptides, antibodies, enzymes, and thiolated polymers via Michael addition to form highly stable thioether bonds. At higher pH values, vinyl sulfone can also react with primary amines, providing additional versatility for biomolecule conjugation.
The PEG spacer enhances aqueous solubility, improves biocompatibility, reduces immunogenicity, minimizes nonspecific protein adsorption, and prolongs circulation time in vivo. mPEG-VS is widely employed in therapeutic protein PEGylation, antibody modification, hydrogel crosslinking, nanoparticle surface engineering, and advanced biomedical research.
Applications
- Protein PEGylation
- Thiol-selective conjugation
- Peptide conjugation
- Antibody modification
- Hydrogel synthesis
- Polymer crosslinking
- Nanoparticle functionalization
- Surface modification
- Drug delivery systems
- Biomaterials research
Features and Benefits
- Linear monofunctional PEG reagent
- Reactive vinyl sulfone functionality
- Efficient thiol-selective Michael addition
- Stable thioether bond formation
- Can react with primary amines under alkaline conditions
- Excellent water solubility
- Excellent biocompatibility
- Available in multiple PEG molecular weights
