Description
General Description
N3-PEG-NHS (Azide-PEG-NHS Ester) is a versatile heterobifunctional PEG linker designed for orthogonal bioconjugation strategies. The terminal NHS ester readily reacts with primary amines on proteins, peptides, antibodies, enzymes, oligonucleotides, and other biomolecules under mild aqueous conditions to form stable amide bonds.
The terminal azide group serves as a bioorthogonal reactive handle for copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC) with strained alkynes such as DBCO and BCN. This combination enables sequential conjugation of two different biomolecules while maintaining excellent reaction selectivity.
The hydrophilic PEG spacer improves water solubility, molecular flexibility, and biocompatibility while reducing nonspecific protein adsorption. N3-PEG-NHS is widely employed in antibody conjugation, protein labeling, nanoparticle engineering, hydrogel synthesis, drug delivery systems, molecular imaging, and advanced biomaterials development.
Applications
- Antibody conjugation
- Protein conjugation
- Peptide conjugation
- Click chemistry conjugation
- Nanoparticle functionalization
- Surface modification
- Hydrogel synthesis
- Drug delivery systems
- Molecular imaging
- Biomaterials research
Features and Benefits
- Heterobifunctional PEG linker
- Reactive NHS ester
- Terminal azide functionality
- Compatible with CuAAC click chemistry
- Compatible with SPAAC chemistry
- Efficient amine-specific conjugation
- Excellent water solubility
- Excellent biocompatibility
- Available in multiple PEG molecular weights
